At that time, the early signals have been dismissed as particles, even so the retrospective analysis shown shared attributes Together with the problematic cells in tradition. Tracing the an infection assisted the lab build supplemental laboratory methods for screening and defense of assay-critical cell lines.
As samples are acquired on the Attune CytPix Flow Cytometer, the large-velocity brightfield camera captures and merchants images of detected activities at a level up to 6,000 pictures for each second, according to the flow level and graphic dimension. For larger overall flexibility, Attune Cytometric Application helps you to change the picture seize frequency as desired and select a certain gate for image seize, if required.
Combine acoustic focusing engineering with large-parameter Investigation for versatile spectral unmixing and standard workflows.
The award-winning Invitrogen Attune CytPix Flow Cytometer combines acoustic concentrating by using a substantial-velocity brightfield digital camera for simultaneous large throughput flow cytometry and large resolution brightfield imaging.
*Pricing is indicative only and it is subject matter to vary. Be sure to Call your Agilent consultant with any thoughts. The information set forth herein is not really a suggestion or maybe a dedication, each of that happen to be issue to credit history acceptance, conditions and terms, and availability with Agilent. Agilent Economical Answers is obtainable in picked nations around the world.
Below, a highly trained person has gated singlets confidently. Right after analyzing the guide singlet gate, the CytPix picture-derived parameter ParticleCount reveals this gate has over 4% aggregates.
Pair your Attune flow cytometer with the autosampler for amplified workflow efficiency, and insert robotic integration for maximum throughput.
Dependable image top quality even at higher throughput Acoustic concentrating and a substantial-pace camera Mix to image these T cells continually at minimal or superior flow charges. Simply regulate concentration and digicam settings to meet experimental specifications.
The Attune NxT cytometer would be the dependable workhorse instrument your lab can depend upon for constant and trustworthy effectiveness. The Attune CytPix design features a substantial-speed brightfield camera and AI-pushed image Evaluation software package, with recent upgrades including equipment Studying tools and an intuitive consumer interface, enabling Increased facts precision, accuracy, and quicker insights into sample biology.
People can view celebration pictures in genuine-time to substantiate sample high quality, enhance protocols, and history the morphology of each and every cell populace discovered for Investigation. Imaging seize also Advantages from acoustic concentrating, which helps to position the cells to ensure that a pointy, centered image is received.
Sound waves help specific mobile alignment, causing Fantastic info high-quality and ~10X more quickly sample flow rates.
We Formerly demonstrated the strength of imaging Vehicle-T/Ramos mobile interactions. Permit’s examine just the populace of biggest curiosity, the double good occasions, to learn more. We could now use extended morphological parameters (here, circularity and skewness of depth) to further more analyze the capabilities of those populations and refine gating on these functions, rising details robustness.
Developed-in dimensionality reduction instruments amplified resolution into populations. Fluorescently gated populations back-gated onto UMAP plot to confirm envisioned inhabitants resolution in lysed whole human blood. User can look at gatherings from Just about every cluster for Visible affirmation of mobile phenotypes.
Versatile payment strategy from Agilent Money Solutions assist you to obtain the crucial instrumentation you need for instant job, or to develop your lab.
At the guts of each and every Attune Flow Cytometer lies our innovative acoustic focusing engineering, which makes use of seem waves to meticulously align cells throughout the flow cell. This revolutionary solution surpasses traditional hydrodynamic methods, causing a lot quicker processing speeds, Fantastic data top quality, and improved sample throughput.
Because the mobile line experienced Beforehand been analyzed within the Attune CytPix Flow Cytometer, the researchers went back to the pictures and were being capable to document the microbial infection a minimum of five times previously.
The Attune CytPix Flow Cytometer permits customers to read more visualize samples through acquisition, verify the accuracy of gating tactics, and assist effects with trustworthy documentation for publication functions.
What appeared to be a homogeneous inhabitants is in fact more varied—an insight that needs to be regarded as when interpreting success.